The somatic cells constitute the set of the material of disintegration of the tissue of the udder and of leukocytes or white blood cells from blood. These elements increase as a result of a state of inflammation, infection or generally udders suffering , (sub-clinical mastitis, micro edema intramammary ,histamine similar reactions, micro-trauma of the canal striatum). The causes are many: feed (mycotoxins, excess of fermentability of both the protein fraction and / or the energy fraction, feed allergens, bacterial toxins introduced with food or produced in the intestines, etc..) Traumatic (defective milking machine, too much hard and inelastic teats cup, causing edema), bacterial (toxins from udder pathogens, environmental germs non-pathogenic but able to produce toxins, etc),genetic, etc.
A high number of cells greater than 150,000 to 200,000 cells per ml of milk represent first alarm that regards: the state of health of the animal and economic aspect of the viability of animal.
Any increase in somatic cells above the limit indicated lose quantity and quality of the milk produced.
Schedule of epidemiological survey
presence of large amounts of biogenic amines, mycotoxins, bacterial toxins in the components of the ration (corn silage and grass, flour, cotton, cereals, bran);
Excess nitrogen fermentable linked to improper intake of fermentable sugars;
Too much fermentable sugars;
Change of feed;
Insufficient intake of long fiber;
Excess of fine particles in the diet.
Milking systems are not perfectly calibrated;
A sheath of the teats is not perfect, too hard or cracked. etc..
Too much individual susceptibility to environmental stresses;
Low resistance to mechanical stress of the teat of the milking;
Altered permeability of blood vessels with excessive passage of white blood cells.
Activation, due to environmental stress or trauma, potentially pathogenic agents characterized by low pathogenicity (subclinical mastitis) or high pathogenicity of microorganisms such as E. coli or S. aureus (acute mastitis).
Admissions poorly designed, inadequate or poorly constructed: wet litter and poorly drained, wet or muddy areas of excess, relationship between the man and animal – farm management.
Cell Line Program
It represents one of the most innovative proposals for an effective program of prevention and treatment of somatic cells due to dietary factors and disease. Born after long years of experience in the field and laboratory employs the Total Fermentation of yeast biomass grown on specific substrates previously hydrolyzed vegetable extracts of Aspergillus oryzae and other microbial derivatives with high enzymatic addition of chelated trace elements immumostimolante action, and plant extracts are characterized by bacteriostatic and bactericidal activity. These elements, which are additions to the metabolic action of yeast, are able to drastically reduce the endogenous production of toxins and inflammatory effect of amine and simultaneously metabolize similar substances introduced with food in addition to increasing the immune response of’ animal.
The mechanism of action of the active ingredients in Cell Line can be explained as:
The presence of biologically active substances derived from fermentation of yeast (Saccharomyces and Kluyveromyces), promotes the power domain of a cellulolytic flora that effectively counteracts the development of flora proteinolitica. In fact, this is the main cause of the production of toxins and biogenic amines like histamine effect. Those toxins arrive at the udder, passing away the liver, alter the permeability of blood vessels resulting in the formation of micro edema borne by the acinar glands (acini) .
In the intestine
The presence of proteins derived from the cell walls of the specific intestinal bacteria are able to stimulate glands muciparous of the intestine and promote an abundant production of immune globulins in nonspecific chick A.These actions increase the thickness of the protective layer intestinal epithelium, improve the metabolic processes in general and increase the ability of the active layer that covers the epithelium, consisting of mucus, IgA and bacteria ,metabolize of any toxins that cross it.The presence of substances having immunostimulant effect of a mineral nature and vegetalete raises the minimal limit of the animal’s response to external stressors. The presence of extracts from medicinal plants exerts a beneficial action to contain the development of many types of friendly bacteria. The use of such products is safe because there is no release of harmful residues for the cheese-making and therefore their use does not include the depreciation of the quality of milk and milk products for use or possible exclusion.
Alveoli – (acini)
Are sack-like structures where milk is synthesized and secreted? An alveolus is the discrete milk producing unit. A single layer of secretory epithelial cells lines the lumen of the alveolus. Contractile myoepithelial cells surround the epithelial lining. Myoepithelial cells contract in response to the hormone oxytocin, resulting in milk being squeezed out of the alveolar lumen and into the small ducts. Outside of the myoepithelial cells the alveolus is surrounded by a connective tissue basement membrane. The capillary bed on the outside of the alveolus is part of the stromal connective tissue between alveoli. A group of alveoli can be visualized as a clump of grapes, with the stems acting as the small ducts leading from the alveoli.
Mechanism of action
The products of the Cell Line are innovative because associated with the typical, long been known, action of metabolic stimulation, carried by:
Saccharomyces cerevisiae cultured on the intestinal flora,
The catalytic properties explicitly on the digestibility of dietary principles from Kluyveromyces fragilis and Saccharomyces cerevisiae ,
Fermented in a “solid state” on appropriate substrates by the addition of extracts from Aspergillus oryzae, Bacillus subtilis and lactobacilli spp.,
Beneficial effects exerted on the containment of saprofit flora of the rumen and intestine by extracts of medicinal plants.
By virtue of their formulation (cell content of yeast, substances with catalytic activity resulting from their fermentation, plant extracts, etc.) increase the digestibility of the ration, decrease the loss of substances still highly fermentable to the colon, maintain balanced development of intestinal microbial populations especially those in cecum-colon tract, decrease the production of toxic catabolites resulting from incorrect intestinal fermentation, which are due to fatigue liver, increased somatic cell count and loss of production.
Moreover, the Cell Line products contain intestinal lactobacilli species specific with all their metabolites, produced during fermentation(organic acids, biocine, peptides with biological action) which are suitably treated in the way to make them non-viable while remaining extremely active in increasing the level of immune response of the animal. Indeed, the presence of proteins of the cell wall undenaturated of lactobacilli is able to stimulate the muciparous cells of the intestinal epithelium to the increased production of mucus and at the same time to increase the production of nonspecific immunoglobulin A (IgA). The formation of a thick covering of epithelial cells constituted by mucus and by immunoglobulin, decreases the possibility that pathogenic microorganisms can adhere to specific sites of attack (lectins), and reduces the passage of toxic substances into the blood system decreasing the inflammatory processes in the liver and the states of toxicosis (liver healthier and lower presence of somatic cells).
The CELL LINE includes Akro Cell powder product for the mass treatment of animals, which is necessary if the somatic cell count of the milk mass is less than 750,000 to 800,000 units
If instead the somatic cells of the milk mass are more than 1.000.000 in this case is nessesary individual treatment with Somato Cell syringe 100 grams to be administered orally in one time or at most to be repeated after 24 hours
Why Use AKRO CELL?
Saccharomyces Cerevisae and Kluyveromyces fragilis for the sake of the greatest yeast feed efficiency in cattle feeding, Akron, based on the results of scientific studies has also developed a technology for the production of inactivated yeast hydrolyzed. The optimum parameters of the hydrolysis process to ensure a high soluble fraction of yeast cells, primarily sugars. Soluble fraction determines the efficiency of the yeast used as a feed additive for dairy cows. A preferred feature of this yeast as compared to other additives is their high stability in the production and storage of feed. Hydrolyzed Yeast have positive effect on the rumen fermentation processes, regardless of the composition of a component of the ration. The results of studies involving hydrolyzed yeast showed that AKRO CELL affected the increased production of volatile fatty acids in the rumen, and the growth of cellulolytic bacteria population, allowing increased digestibility of roughage. Furthermore, the considerable increase of the microbial groups Selenomonas that convert lactic acid to propionic acid, thereby preventing the occurrence of acidosis. As a result, increased the amount of energy used for milk production.
In some cases the rumen environment does not provide optimal conditions for the survival and development of living yeast cells.Inappropriate rumen pH, temperature, oxygen availability, high masticatory competing microorganisms, and short lifetime of yeast cause that they are not able to produce adequate quantities of metabolites.
Metabolites, (the products of yeast metabolism) are used for feeding the bacterial flora in the rumen. That means that AKRO CELL includes not only yeast but their metabolites which are produced by fermentation of the yeast. Some of these metabolites improves the palatability of feed, which translates into an increase in the amount of dry matter consumed ration. Some metabolites nourishing microflora stabilize and shape the balance between the different populations of rumen bacteria and promote faster growth of bacteria cellulolytic which are involved in the digestion of roughage. This increases the production of volatile fatty acids in the rumen, which is the energy source for the production of milk. The consequence of a well-functioning rumen is increased milk production with inter alia: fewer somatic cells.
Extract of Aspergillus oryzae—provides active enzymes and stimulates rumen microbes.
The enzyme complex (amylase, protease, cellulase) derived from a fermentation process, which will improve the solubility of the nutritional supplements the most resistant for the endogenous enzymes attack. Digestive enzymes of plant origin are derived from the fungus Aspergillus oryzae which:
• complement deficiency of digestive enzymes
• regulate the digestive system (brings positive results on various problems with digestion)
• brings the optimal ability of digestive
• indirectly increase the efficiency of the immune systemilize rumen pH, and reduce heat stress.
Bacillus subtilis and lactobacilli One and very interesting from the point of view of the mechanisms, as well as clinically important field of application of probiotics is their ability to inhibit bacterial translocation. The aim of these tests is stimulated by probiotic change in the composition of the intestinal flora, decreasing number of pathogenic bacteria and improve intestinal mucosal barrier function.
Probiotic bacteria also possess immunomodulatory properties. These characteristics, resulted attempts to apply the bacteria of the genera Lactobacillus, Bacillus, and cells of the yeast Saccharomyces, as adjuvants and carriers of other antigens used for immunization. Due to the huge variety of different strains of probiotic bacteria on the immune system, can induce various forms an immune response in the individual circuits of the system.
Lactobacillus plantarum has demonstrated improvement in cases of enteric bacterial infections. This bacterium reinforces the barrier function of the intestinal mucosa, thus preventing the attachment of harmful bacteria. Significant benefits include:
•Preserving key nutrients, vitamins, and antioxidants
•Manufacturing vitamins B1, B2, B3, B5, B6, B12, A and K, and short chain fatty acids
•Contributes to the destruction of moulds, viruses, and parasites
•Increasing the number of immune system cells
• promotes the breakdown and assimilation of nutrients.
•Reducing toxic waste at the cellular level
•Stimulating the repair mechanism of cells
•Synthesizing the anti-viral amino acid, L-lysine
•Eliminating toxic components from food including nitrates
Betaine improves the functioning of the liver and prevents the risk of fatty.
Niacin (vitamin PP) Coenzyme system in biological reactions, improve energy balance in early lactation cows, minimize ketosis, and stimulate rumen protozoa.
Zinc improves immune response, harden hooves, and lower somatic cell counts.
Extracts of medicinal plants
Carvacrol strong bacteriostatic compounds of natural origin that can both metabolism – better digestion and absorption of nutrients, reduce the incidence of pathogenic bacteria and stabilization of microflora in the gastrointestinal tract, as well as increasing the production and secretion of enzymes and digestive juices.
Spiraea ulmaria Flower extracts help the body remove harmful metabolites, acts bactericidal and has a high antibacterial activity.
Salix alba contains phenolic glycosides (including salicin), flavonoids, organic acids and mineral salts. Works inflammatory.
Echinacea The secret lies in the effectiveness of echinacea beneficial ingredients. Are especially important polysaccharides, which stimulate the immune system, which contributes to the increasing resistance. Activated macrophages and leukocytes, which secrete antiviral substances.
Eleutherococcus activates the body’s natural defenses. Has adaptogenic properties, which act on the body as a whole by restoring balance at all levels. An adaptogen is a lot of medicinal substances in a single plant, used in accordance with the current state of the body. Adaptogens mobilize the necessary ingredients provide natural forces.
Mastitis prevention agent;
AKRO CELL prevents mastitis by strengthening the tissues in mammary gland and has an outstanding effect as an anti-inflammatory agent.
Increase in milk production;
AKRO CELL increases digesting capability and absorption rate and shows an absolutely different capability in milk production from before.
Increase in immunity;
AKRO CELL assists in colonizing the intestine with favorable bacteria, suppresses pathogenic bacteria, and produces digestive enzymes.
Increased activity of metabolism of toxic substances in the mucus layer of the intestinal epithelium by the wall proteins of specific lactobacillus, able to stimulate the epithelium.Decrease in the quantity of histamine similar substances which pass into the circulation
Increasing the availability of propionate and lactate by effect of stimulating the ruminal fermentation due to the presence of Total Fermentation Biomass of Saccaromyces and Kluyveromyce;
AKRO CELL by Propionibacterium helps to convert lactate to propionate and stabilize rumen pH in high carbohydrate diets. Also stimulates feed intake.
Improvement of intestinal absorption as a result of the balance of the intestinal microflora;
Better ruminal digestion, metabolism and improved nutrient utilization. Increase rate of fiber digestion (thus directly affecting gut fill) and the rate of digesta flow. Aspergillus oryzae provides active enzymes and stimulates rumen microbes. Effect buffer indirect, on rumen pH, for preferential stimulation of specific classes microbial.
UTILIZATION AND DOSES
Administer AKRO CELL to dairy cows in the ordinary feed in the way to administer the following dosages:
During the first 10 days: 70 g head / day. Following: 30 g head / day
After (until somatic cell count return to normality): 40 g/head/day.